Figure 1

Effect of mesenchymal stem cell infusion in multiple myeloma model mice. (A) Experimental protocol of multiple myeloma (MM) cell and mesenchymal stem cell (MSC) injection and the design of lymphocyte Peyer’s patch adhesion molecule (LPAM) administration. (B) Six-week survival rates of animals in different groups (for each group, n = 14). The survival rate in the nontreated MM model group was dramatically decreased after 3 weeks. The MSC and L-PAM groups had almost the same survival rate, but were higher than the MM group (P <0.05). Data shown as the mean ± standard deviation (SD) of three parallel experiments. *P <0.05 versus MM group. (C) Comparison of clinical findings in each group. The number (left) and size (right) of cancroid pearls in the neck, root of tail and abdominal cavity are shown as column graphs (top panel). Data shown as mean ± SD for three parallel experiments. *P <0.05 versus MSC group; #P <0.05 versus L-PAM group. Lower panel: representative photograph of typical cancroid pearls in the base of the tail (bar = 5 mm). (D) Comparison of bone resorption in lumbus and collum femoris in each group. Radiographs of lumbus and collum femoris at 3 weeks after MM cell injection, and the bone density in each group was analyzed. Data shown as mean ± SD for four parallel experiments. *P <0.05 versus MM group; #P <0.05 versus L-PAM group. (E) Myeloma cell metastasis in the lungs and kidneys in each group. Data shown as mean ± SD for three parallel experiments. *P <0.05 versus MSC group. Left panels: light micrographs of the lungs and kidneys (hematoxylin and eosin staining, bar = 100 μm). Con, control.