Figure 5

Effect of mesenchymal stem cells with highly activated Fas ligand on multiple myeloma model mice. (A) Five-week survival rates of multiple myeloma (MM) model mice. (B) Comparison of the number (left) and size (right) of cancroid pearls in MM model mice after treatment with mesenchymal stem cells (MSCs) expressing different levels of Fas ligand (Fas-L). Data presented as mean ± standard deviation (SD) for three parallel experiments. *P <0.05 versus MSC group; #P <0.05 versus the control group. Right panel: representative photograph of typical cancroid pearls at the base of the tail (bar = 5 mm). (C) Distribution of cancroid pearl size in the four test groups (n = 14). (D) Apoptosis of MM cells in MM model mice. The pearls in each group were triple-stained for 4′,6-diamidino-2-phenylindole ( blue), Scar-1 (green) and Annexin V/7AAD (red). All groups treated with MSCs had Scar-1-positive cells in the pearls. However, the positive reactions of apoptosis marker in the MSC(gld) group were much lower than those observed in other groups. The MSC(Asp) group had the highest number of reactions among all groups (bar = 5μm). Lower panels stained through the terminal deoxynucleotidyl transferase-mediated UTP nick-end labeling (TUNEL) assay. Positive reactions were observed in both MSC and MSC(Asp) groups. ASP, aspirin; Con, control; gld, generalized lymphoproliferative disease.