Figure 3

In vitro osteogenic, adipogenic and chondrogenic differentiation of bone marrow mesenchymal stromal cells. Bone marrow-derived mesenchymal stromal cells (BMMSCs; passage 2 to 3) were cultured in vitro under osteogenic, adipogenic and chondrogenic induction condition for 3 weeks. Alizarin Red staining shows mineralization deposition, Oil Red O staining demonstrates the generation of lipid-containing adipocytes and Alcian blue staining demonstrates cartilage matrix (magnification 400). Data shown are representative of six independent experiments.