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Table 1 Differential gene expression between bone marrow-derived hematopoietic stem/progenitor cells and bone marrow-derived mesenchymal stromal cells

From: Comparative analysis of gene transcripts for cell signaling receptors in bone marrow-derived hematopoietic stem/progenitor cell and mesenchymal stromal cell populations

Gene identification

Assay on demanda

∆Ct HSPCb

∆Ct BMMSCb

Fold-change HSPC/BMMSCc

P value

RTK gene transcripts

     

Aatk-apoptosis-associated tyrosine kinase

Mm00545697_m1

16.8 ± 0.57

17.91 ± 0.30

2.5

0.009

Axl-AXL receptor tyrosine kinase

Mm00437221_m1

21.03 ± 0.41

11.49 ± 0.32

-818.7

0.0001

Mertk-c-mer proto-oncogene tyrosine kinase

Mm00434920_m1

23.04 ± 0.76

18.68 ± 0.41

-21.7

0.0001

Tyro3-TYRO3 protein tyrosine kinase 3

Mm00444547_m1

21.49 ± 0.62

15.83 ± 0.38

-52

0.0001

Ddr1-discoidin domain receptor family, member 1

Mm00432251_m1

ND

21.20 ± 0.49

  

Ddr2-discoidin domain receptor family, member 2

Mm00445615_m1

ND

11.46 ± 1.94

  

Egfr-epidermal growth factor receptor

Mm00433023_m1

23.44 ± 0.64

16.89 ± 0.48

-88.8

0.0001

Epha1-Eph receptor A1

Mm00445804_m1

23.88 ± 0.83

20.73 ± 0.42

-9.2

0.0001

Epha2-Eph receptor A2

Mm00438726_m1

19.20 ± 0.96

17.34 ± 0.46

-3.8

0.0001

Epha3-Eph receptor A3

Mm00580743_m1

ND

22.18 ± 0.33

  

Epha4-Eph receptor A4

Mm00433056_m1

22.17 ± 0.49

20.76 ± 0.41

-2.8

0.0001

Epha6-Eph receptor A6

Mm00433094_m1

ND

ND

  

Epha7-Eph receptor A7

Mm00833876_m1

22.06 ± 1.10

17.57 ± 0.29

-22.9

0.0001

Epha8-Eph receptor A8

Mm00433106_m1

ND

ND

  

Ephb3-Eph receptor B3

Mm00802553_m1

ND

16.69 ± 0.79

  

Ephb4-Eph receptor B4

Mm00438750_m1

22.21 ± 0.46

15.50 ± 0.33

-107.3

0.0001

Fgfr1-fibroblast growth factor receptor 1

Mm00438923_m1

20.91 ± 1.27

15.95 ± 0.20

-36.5

0.0001

Fgfr2-fibroblast growth factor receptor 2

Mm00438941_m1

ND

20.55 ± 0.40

  

Fgfr3-fibroblast growth factor receptor 3

Mm00433294_m1

ND

20.32 ± 0.48

  

Insr-insulin receptor

Mm00439693_m1

15.97 ± 0.43

15.82 ± 0.29

-1.1

0.3273

Met-met proto-oncogene

Mm00434924_m1

18.25 ± 0.38

14.36 ± 0.49

-15.9

0.0001

Mst1r-macrophage stimulating 1 receptor (c-met-related tyrosine kinase)

Mm00436365_m1

ND

24.49 ± 1.08

  

Musk-muscle, skeletal, receptor tyrosine kinase

Mm00448006_m1

ND

ND

  

Csf1r-colony stimulating factor 1 receptor

Mm00432689_m1

14.89 ± 0.43

22.45 ± 0.43

197.3

0.0001

Flt3-FMS-like tyrosine kinase 3

Mm00438996_m1

17.68 ± 0.87

ND

  

Kit-kit oncogene

Mm00445212_m1

15.21 ± 0.63

ND

  

Pdgfra-platelet derived growth factor receptor, alpha polypeptide

Mm00440701_m1

ND

16.71 ± 0.66

  

Pdgfrb-platelet derived growth factor receptor, beta polypeptide

Mm00435546_m1

19.98 ± 0.60

13.83 ± 0.24

-72.4

0.0001

Ret-ret proto-oncogene

Mm00436304_m1

22.27 ± 0.64

20.56 ± 0.41

-3.4

0.0001

Ror1-receptor tyrosine kinase-like orphan receptor 1

Mm00443462_m1

ND

18.75 ± 0.37

  

Ror2-receptor tyrosine kinase-like orphan receptor 2

Mm00443470_m1

ND

19.22 ± 0.30

  

Ros1-Ros1 proto-oncogene

Mm00803362_m1

ND

ND

  

Tek-endothelial-specific receptor tyrosine kinase

Mm00443242_m1

18.52 ± 1.21

ND

  

Tie1-tyrosine kinase receptor 1

Mm00441786_m1

20.39 ± 0.84

ND

  

Ntrk2-neurotrophic tyrosine kinase, receptor, type 2

Mm00435422_m1

ND

ND

  

Flt1-FMS-like tyrosine kinase 1

Mm00438980_m1

21.13 ± 0.41

20.52 ± 0.49

-1.6

0.0032

Kdr-kinase insert domain protein receptor

Mm00440099_m1

23.48 ± 0.60

23.31 ± 0.70

-1.3

0.5296

Non-RTK transcripts

     

Abl1-v-abl Abelson murine leukemia oncogene 1

Mm00802038_g1

16.54 ± 0.54

14.93 ± 0.14

-3

0.0001

Tnk1-tyrosine kinase, non-receptor, 1

Mm00840782_g1

ND

ND

  

Tnk2-tyrosine kinase, non-receptor, 2

Mm00450301_m1

17.25 ± 0.34

15.88 ± 0.25

-2.6

0.0001

Matk-megakaryocyte-associated tyrosine kinase

Mm00440268_m1

17.49 ± 0.83

22.68 ± 0.58

41.9

0.0001

Ptk2-PTK2 protein tyrosine kinase 2

Mm00433209_m1

20.21 ± 0.90

14.67 ± 0.28

-47.1

0.0001

Fert2-fer (fms/fps related) protein kinase, testis specific 2

Mm00484303_m1

17.74 ± 0.48

15.11 ± 0.22

-6.3

0.0001

Fes-feline sarcoma oncogene

Mm00802572_g1

13.51 ± 0.47

17.67 ± 0.45

18.8

0.0001

Frk-fyn-related kinase

Mm00456656_m1

ND

18.56 ± 0.54

  

Srmssrc-related kinase lacking C-terminal regulatory tyrosine

Mm00441546_m1

ND

ND

  

Jak1-Janus kinase 1

Mm00600614_m1

13.23 ± 0.54

12.47 ± 0.43

-1.8

0.0009

Jak2-Janus kinase 2

Mm00434561_m1

14.69 ± 0.63

15.81 ± 0.25

2.4

0.0001

Jak3-Janus kinase 3

Mm00439962_m1

16.78 ± 0.62

17.93 ± 0.20

2.4

0.0001

Fgr-Gardner-Rasheed feline sarcoma viral (Fgr) oncogene homolog

Mm00438949_m1

14.67 ± 1.61

ND

  

Fyn-Fyn proto-oncogene

Mm00433373_m1

17.01 ± 0.54

14.57 ± 0.40

-5.6

0.0001

Hck-hemopoietic cell kinase

Mm00439302_m1

14.2 ± 0.76

22.11 ± 0.54

275.1

0.0001

Lck-lymphocyte protein tyrosine kinase

Mm00802897_m1

19.42 ± 0.49

23.04 ± 0.43

13

0.0001

Lyn-Yamaguchi sarcoma viral (v-yes-1) oncogene homolog

Mm00802933_m1

13.28 ± 0.87

19.56 ± 0.51

93.1

0.0001

Bmx-BMX non-receptor tyrosine kinase

Mm00515368_m1

14.73 ± 1.07

ND

  

Btk-Bruton agammaglobulinemia tyrosine kinase

Mm00442712_m1

15.05 ± 1.20

24.92 ± 0.76

972.4

0.0001

Tec-cytoplasmic tyrosine kinase, Dscr28C related (Drosophila)

Mm00443230_m1

16.2 ± 0.34

18.24 ± 0.33

4.2

0.0001

Txk-TXK tyrosine kinase

Mm00443280_m1

20.98 ± 0.50

ND

  

Syk-spleen tyrosine kinase

Mm00441649_m1

12.74 ± 0.57

17.10 ± 0.21

22.1

0.0001

Zap70-zeta-chain (TCR) associated protein kinase

Mm00494255_m1

19.49 ± 1.30

ND

  

G-protein coupled receptor transcripts

     

Agtrl1-angiotensin receptor-like 1

Mm00442191_s1

19.96 ± 0.56

20.22 ± 0.88

1.3

0.3972

Ednrb-endothelin receptor type B

Mm00432989_m1

22.96 ± 1.06

22.70 ± 0.67

-1

0.4802

Ccr1-chemokine (C-C motif) receptor 1

Mm00438260_s1

13.33 ± 0.63

19.78 ± 1.13

95.6

0.0001

Ccr3-chemokine (C-C motif) receptor 3

Mm00515543_s1

19.93 ± 0.36

20.03 ± 1.10

1.1

0.7675

Ccr4-chemokine (C-C motif) receptor 4

Mm00438271_m1

23.9 ± 1.24

ND

  

Ccr7-chemokine (C-C motif) receptor 7

Mm00432608_m1

18.32 ± 1.50

ND

  

Ccr8-chemokine (C-C motif) receptor 8

Mm00843415_s1

21.55 ± 0.44

19.71 ± 0.83

-4.1

0.0001

Cxcr3-chemokine (C-X-C motif) receptor 3

Mm00438259_m1

20.74 ± 0.36

ND

  

Blr1 (Cxcr5)-Burkitt lymphoma receptor 1

Mm00432086_m1

20.88 ± 1.00

ND

  

Cmkor1 (Cxcr7)-chemokine orphan receptor 1

Mm00432610_m1

23.68 ± 1.01

15.52 ± 0.35

-293.2

0.0001

Cytokine receptor transcripts

     

Acvr1-activin A receptor, type 1

Mm00431645_m1

17.98 ± 0.83

13.57 ± 0.45

-22.2

0.0001

Acvrl1-activin A receptor, type II-like 1

Mm00437432_m1

20.73 ± 0.60

19.32 ± 0.67

-2.9

0.0001

Bmpr1a-bone morphogenetic protein receptor, type 1A

Mm00477650_m1

17.28 ± 0.95

14.75 ± 0.22

-5.9

0.0001

Il3ra-interleukin 3 receptor, alpha chain

Mm00434273_m1

18.52 ± 0.53

18.40 ± 0.44

-1.1

0.5524

Il6ra-interleukin 6 receptor, alpha

Mm00439653_m1

14.54 ± 0.38

16.40 ± 0.51

3.8

0.0001

Il7r-interleukin 7 receptor

Mm00434295_m1

17.11 ± 0.24

24.38 ± 0.75

155.9

0.0001

Csf2ra-colony stimulating factor 2 receptor, alpha

Mm00438331_g1

14.46 ± 0.40

19.04 ± 0.22

24.9

0.0001

Csf2rb2-colony stimulating factor 2 receptor, beta 2

Mm00655763_m1

17.33 ± 0.38

ND

  

Csf3r-colony stimulating factor 3 receptor

Mm00432735_m1

11.85 ± 1.43

ND

  

Epor-erythropoietin receptor

Mm00833882_m1

19.42 ± 1.07

22.59 ± 0.85

11.6

0.0001

Lifr-leukemia inhibitory factor receptor

Mm00442940_m1

17.89 ± 0.64

16.76 ± 0.36

-2.3

0.0001

Mpl-myeloproliferative leukemia virus oncogene

Mm00440310_m1

14.86 ± 1.69

ND

  

Ngfr-nerve growth factor receptor (TNFR superfamily, member 16)

Mm00446294_m1

ND

11.96 ± 0.30

  

Osmr-oncostatin M receptor

Mm00495424_m1

ND

15.97 ± 0.24

  

Tnfrsf1a-tumor necrosis factor receptor superfamily, member 1a

Mm00441875_m1

13.49 ± 0.36

13.91 ± 0.24

1.4

0.0023

Other signaling molecule transcripts

     

Abcb1b-ATP-binding cassette, sub-family B (MDR/TAP), member1B

Mm00440736_m1

18.64 ± 0.64

17.73 ± 0.38

-1.9

0.0003

Mrc1-mannose receptor, C type 1

Mm00485148_m1

20.76 ± 0.47

22.00 ± 0.62

2.5

0.0001

Gata4-GATA binding protein 4

Mm00484689_m1

ND

20.10 ± 0.31

  

Hoxb4-homeo box B4

Mm00657964_m1

17.88 ± 0.50

17.28 ± 0.59

-1.7

0.0134

Ptprc-protein tyrosine phosphatase, receptor type, C

Mm00448463_m1

ND

ND

  

Gas6-growth arrest specific 6

Mm00490378_m1

23.45 ± 1.02

14.90 ± 0.26

-381.1

0.0001

Lgals9-lectin, galactose binding, soluble 9

Mm00495295_m1

13.67 ± 0.82

17.69 ± 0.25

18.9

0.0001

Sca1-spinocerebellar ataxia 1 homolog (human)

Mm00485928_m1

17.08 ± 0.23

15.71 ± 0.29

-2.6

0.0001

Spp1-secreted phosphoprotein 1

Mm00436767_m1

17.91 ± 0.33

9.23 ± 0.44

-424.9

0.0001

  1. Gene differential expression was considered significant with P <0.05. Mean ± standard deviation of six independent HSPC and BMMSC preparations are shown. BMMSC, bone marrow-derived mesenchymal stromal cell; Ct, cycle threshold; HPSC, hematopoietic stem/progenitor cell; ND, not detectable; RTK, receptor tyrosine kinase. aQuantitative PCR was performed on an ABI PRISM 7900HT Sequence Detection System using a custom-made TaqMan® Low Density Array. mRNA transcripts were evaluated with TaqMan® Probes commercially available as Assay on Demand (Applied Biosystems, Foster City, CA, USA) with optimized primer and probe concentrations.
  2. bMean ± standard deviation mRNA level of each gene in each cell population (six individual cell samples run in duplicate) were first normalized to the expression of 18S RNA in that sample.
  3. cMean fold-changes in gene transcript expression levels between HSPCs and BMMSCs were evaluated with 2–ΔΔCt.
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Stem Cell Research & Therapy

ISSN: 1757-6512