Figure 6

MSCs exposed to tumor CM lose their multipotent differentiation potential. (a) Control MSCs or MSCs exposed to FaDu or MDA-MB-231 CM (10 days) were harvested and seeded on top of Matrigel® as indicated in Methods sections. Vessel-like tubular formation was assessed at 2 hours and 72 hours using a fluorescence microscope at 4x and 10x, as indicated. Data are representative of at least two experiments. Control MSCs or MSCs exposed to FaDu or MDA-MB-231 CM (10 days) were switched to adipogenic (b) or osteogenic induction media (c). On day 6, adipocyte differentiation was measured using Oil-Red-O staining (b), while osteoblast differentiation was measured using alkaline phosphatase staining (ALP) (c). Data are representative of at least two experiments. CM, conditioned media; MSCs, mesenchymal stem cells.