Table 1 PCR Probes and primers of nucleus pulposus markers and corresponding antibodies for immunostaining for protein expression
Target | PCR probe/primers | Antibody | Â |
|---|---|---|---|
| Â | Order number | Vendor (order number) | Host/type |
Aggrecan | Hs 00153936_m1 | NA | NA |
Type II collagen | Hs 00156568_m1 | Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA, USA (II-II6B3) | Ms/monoclonal |
Integrin α3 | Hs 01076873_m1 | Chemicon, Temecula, CA, USA (AB1920) | Rb/polyclonal |
Integrin α6 | Hs 00173952_m1 | BD Biosciences, San Jose, CA, USA (555734) | Rat/monoclonal |
Integrin β4 | Hs 00173995_m1 | Chemicon (AB1922) | Rb/polyclonal |
CD239 (Lu) | Hs 00170663_m1 | AbD Serotec, Oxford, UK (MCA1982) | Ms/monoclonal |
Laminin α5 | NA | Chemicon (MAB1924) | Ms/monoclonal |