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Table 1 PCR Probes and primers of nucleus pulposus markers and corresponding antibodies for immunostaining for protein expression

From: Human umbilical cord mesenchymal stromal cells exhibit immature nucleus pulposus cell phenotype in a laminin-rich pseudo-three-dimensional culture system

Target PCR probe/primers Antibody  
  Order number Vendor (order number) Host/type
Aggrecan Hs 00153936_m1 NA NA
Type II collagen Hs 00156568_m1 Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA, USA (II-II6B3) Ms/monoclonal
Integrin α3 Hs 01076873_m1 Chemicon, Temecula, CA, USA (AB1920) Rb/polyclonal
Integrin α6 Hs 00173952_m1 BD Biosciences, San Jose, CA, USA (555734) Rat/monoclonal
Integrin β4 Hs 00173995_m1 Chemicon (AB1922) Rb/polyclonal
CD239 (Lu) Hs 00170663_m1 AbD Serotec, Oxford, UK (MCA1982) Ms/monoclonal
Laminin α5 NA Chemicon (MAB1924) Ms/monoclonal
  1. Probes and primers from Applied Biosystems (Foster City, CA, USA) of nucleus pulposus markers analyzed by real-time PCR and corresponding antibodies used for immunostaining for protein expression of these markers. Rb, rabbit; Ms, mouse; NA, not applicable; Lu, Lutheran glycoprotein.