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Table 2 Undifferentiated HUCMSCs express cell surface markers typical to MSCs and immature nucleus pulposus cells

From: Human umbilical cord mesenchymal stromal cells exhibit immature nucleus pulposus cell phenotype in a laminin-rich pseudo-three-dimensional culture system

 

Passage 3

Passage 5

Target

% (+) cells

MFI

% (+) cells

MFI

CD44

99

2758

99

917

CD90

99

366

99

120

CD166

98

216

99

53

CD105

75

16

26

9

CD29 (ITGβ1)

99

215

99

692

CD49c (ITGα3)

99

127

99

211

CD49f (ITGα6)

63

34

0

0

CD104 (ITGβ4)

1

12

2

2

CD239 (Lu)

30

17

82

32

CD34

0

0

0

0

CD31

0

0

1

1

CD45

0

0

0

0

  1. Values represented a mean of duplicate samples (n = 2). %(+), percentage of total number of cells expressing the markers. HUCMSC, human umbilical cord mesenchymal stromal cell; ITG, integrin; Lu, Lutheran glycoprotein; MFI, mean fluorescence intensity; MSC, mesenchymal stem cell.
  2. Data were obtained via flow cytometry analysis.