Figure 4

miR-20b inhibited tissue factor expression. (A), (B) Tissue factor (TF)-3′-untranslated region (UTR)-luciferase reporter assay. Various miRNA mimics with either (A) wild-type or (B) mutant TF-3′-UTR-luciferase reporter gene were transfected into granulocyte–macrophage (G-M) cells and trophoblasts, respectively. Then 48 hours post transfection, luciferase activity was measured and reported as the mean ± standard error of the percentages of the luciferase activity in cells without miRNA mimics. (C), mRNA and protein analysis in G-M cells and trophoblasts transfected with various miRNA mimics. (D) TF, PU.1, and CDX2 mRNA levels in G-M cells and trophoblasts transfected with miR-20b inhibitor were analyzed using quantitative real-time polymerase chain reaction and reported as the mean ± standard error of the percentage of the mRNA levels of their corresponding gene in control cells, respectively. *P <0.05. bp, base pair; NC.