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Table 2 Primer pairs used for quantitative real-time polymerase chain reaction

From: MicroRNA-20b and ERK1/2 pathway independently regulate the expression of tissue factor in hematopoietic and trophoblastic differentiation of human embryonic stem cells

Gene symbol

Forward primer (5′ to 3′)

Reverse primer (5′ to 3′)

PU.1

CCTGTATGTAGCGCAAGAGATTTA

CCAGCACAAGTTCCTGATTTTATC

CDX2

AGGGGGTGGTTATTGGACTC

CATTCAGCCCAGAGAAGCTC

TF

GCCAGGAGAAAGGGGAAT

CAGTGCAATATAGCATTTG

GAPDH

ACAGTCAGCCGCATCTTCTT

ACGACCAAATCCGTTGACTC

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Stem Cell Research & Therapy

ISSN: 1757-6512