| Â | Group | Normal area (%) | Alveolar collapse (%) | Hyperinflation (%) |
|---|
| Â | Â | LPS | CLP | LPS | CLP | LPS | CLP |
|---|
|  | C (LPS) or sham (CLP) | 99.6 ± 0.7 | 99.0 ± 1.2 | 0.4 ± 0.7 | 1.0 ± 1.2 | 0.0 ± 0.0 | 0.0 ± 0.0 |
Day 1 | ARDS | 85.0 ± 7.0* | 92.0 ± 3.3* | 13.4 ± 6.0* | 5.6 ± 4.0* | 1.6 ± 3.0 | 0.0 ± 0.0 |
Day 3 | ARDS-SAL | 93.5 ± 2.4* | 86.1 ± 4.2* | 6.5 ± 2.4* | 11.0 ± 1.7* | 0.0 ± 0.0 | 2.9 ± 4.2* |
|  | ARDS-BMDMC | 97.4 ± 1.5 | 94.6 ± 2.5# | 2.6 ± 1.5 | 4.6 ± 2.4# | 0.0 ± 0.0 | 0.0 ± 0.0 |
Day 7 | ARDS-SAL | 94.0 ± 1.0* | 93.6 ± 2.9* | 4.8 ± 2.0* | 6.5 ± 2.8* | 0.0 ± 0.0 | 0.3 ± 1.2 |
|  | ARDS-BMDMC | 98.8 ± 0.8 | 96.8 ± 2.2 | 1.2 ± 0.8 | 3.2 ± 2.2 | 0.0 ± 0.0 | 0.0 ± 0.0 |
- Mice received Escherichia coli lipopolysaccharide (LPS) intraperitoneally or were subjected to cecal ligation and puncture (CLP) surgery. Control group animals received saline intraperitoneally, whereas a sham- operated group was used as control for animals undergoing CLP. At day 1, some animals were sacrificed, post acute respiratory distress syndrome (ARDS), to evaluate lung morphometry, while other animals were further randomized into subgroups to receive saline (SAL) or bone marrow-derived mononuclear cells (BMDMC, 2×106 cells) intravenously. Values expressed as mean ± standard deviation of sic animals in each group. All values were computed in 10 random, noncoincident fields per animal. *Significantly different from corresponding control group (control or sham) (P <0.05). #ARDS-BMDMC vs. ARDS-SAL (P <0.05).
