Figure 2

Transplantation of GFP-transfected hAECs into sterilized recipient mice and the follicle activation induced by hAECs transplantation. (A) hAECs cells grown to 85% density. (B) GFP-transfected hAECs. Representative H&E micrographs of ovary sections from: non-sterilized normal control mice (C, D); sterilized non-transplanted mice after a 7-day recovery period (E), a 14-day recovery period (F), a 21-day recovery period (G) and a 2-month recovery period (H) showing stroma, and atretic primordial or primary follicles; sterilized recipient mice 7 days (I), 14 days (J), 21 days (K) and 2 months (L) after transplantation of hAECs. No obvious follicles were observed in recipients seven days after hAEC transplantation (I); however, the hollow structure destroyed by chemotherapy was reduced and obviously compared with (E). Primordial follicles are visible in J, primary follicles were visible in K and large antral follicles are shown in L. Arrows indicated follicles at various stages of maturational development. Scale bars = 100 μm. (M). Differential follicle counts of primordial, primary, secondary and atretic follicles in ovaries of each groups. NC, Normal control; UC, Untreated control; hAECs, hAECs transplantation group. GFP, Green fluorescent protein; hAECs, Human amniotic epithelial cells.