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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Human amniotic epithelial cells can differentiate into granulosa cells and restore folliculogenesis in a mouse model of chemotherapy-induced premature ovarian failure

Figure 4

Double-staining with GFP and human nuclear antigen to observe GFP-positive cells after hAECs transplantation. (A-F) Ovarian sections stained with GFP and anti-human nuclear antibody reveal grafted hAECs 28 days after hAEC transplantation. (G-L) GFP staining co-localized with human nuclear antigen in antral follicles of recipient ovaries two months after hAEC transplantation. Arrows indicate a double-staining pattern in ovaries. Scale bars, 200 μm (G-L), 100 μm (A-F). GFP, Green fluorescent protein; hAECs, human amniotic epithelial cells.

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