Figure 5

Grafted cells detected by immunochemistry against human antigens. (A) Human specific nuclear antigen expression was negative in recipient ovaries without hAECs transplantation. (B) Human nuclear antigen was not detected in some recipient ovaries two months after hAEC transplantation. (C, D) Human nuclear antigen was expressed in antral follicles in recipient ovaries two months after hAEC transplantation. (E) Human FSHR was not detected in recipient ovaries without hAEC transplantation. (F) Human FSHR was not detected in some recipient ovaries two months after hAEC transplantation. (G, H) Human FSHR were detected in recipient ovaries two months after hAEC transplantation. Arrows indicate human antigen expression in granulosa cells surrounding the ovum, whereas other granulosa cells were negative controls. Scale bars = 100 μm. hAECs, human amniotic epithelial cells.