Immunophenotypic characterization, cell viability, proliferation and differentiation of SiMAG-labelled mMSCS. mMSCs labelled with (C, D) and without (A, B) SiMAG underwent successful differentiation down osteogenic (A, C) and adipogenic (B, D) lineages after 21 days. mMSCs labelled with 10 μg/ml of SiMAG characterised at 24 hours and 7 days by E) MTT analysis and F) live dead staining. *** Indicates P <0.001. Bars = 100 μm. (G) SiMAG-labelled and unlabelled mMSCs were negative for hematopoietic markers CD11b and CD45, endothelial cell marker CD31 and positive for CD44, CD105and Sca-1. The expression of every antigen is shown together with their corresponding isotype control. mMSCs, murine mesenchymal stem cells.