Figure 1

hMSCs inhibit antigen-induced murine T-cell proliferation. A total of 2 × 10⁶ purified antigen specific murine CD4⁺(A) and CD8⁺ T cells (B) were activated by their cognate peptides in the presence of antigen-presenting cells (APCs), and then co-cultured with human bone marrow-derived mesenchymal stem cells (hMSCs) in two different ratios (10:1 and 5:1). T cells were CFSE labeled on Day 0 and then harvested 72 hours later and analyzed by FACS. TCR transgenic CD4⁺ T cells (BDC2.5 T cells) were first gated using CD4 and TCR Vβ4 double staining, while CD8⁺ TCR transgenic CD8 T cells (8.3 T cells) were gated using CD8 and TCR Vβ8.1/8.2 double staining. The gates used to determine the percentage of undivided T cells (right hand bars) and divided T cells (left hand bars) are shown in each panel. The graphs are representative of four different individual experiments with similar results.