Figure 3

hMSCs effect on different activation markers on murine antigen-specific T cells. Murine CD4 (A) and CD8 (B) T cells were isolated and purified from lymph nodes and spleens of TCR transgenic mice using Miltenyi mouse CD4⁺ T cell/CD8⁺ T cell isolation kit and cultured for three days in the presence of specific peptides and APCs. Human bone marrow-derived mesenchymal stem cells (hMSCs) were added to the wells at the beginning of the culture in two ratios (1:10 and 1:5 hMSC to T cells). The cells were harvested and stained with antibodies specific for activation markers as indicated below each panel. Each graph shows overlays of histograms representing each condition as shown in the key: unstimulated T cells - red; stimulated T cells - blue; stimulated T cells + hMSC (ratio 10:1) - green; stimulated T cells + hMSC (ratio 5:1) - orange. The bar graphs depict the mean fluorescence intensity (MFI) of the analyzed surface markers on T cells in different treatment groups. All graphs are representative of three individual experiments with similar results.