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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Assessment of immunosuppressive activity of human mesenchymal stem cells using murine antigen specific CD4 and CD8 T cells in vitro

Figure 4

hMSCs also inhibit the activation of T cells stimulated with anti-CD3/anti-CD28 beads. Mouse Ag specific CD8+ T cells (8.3 T cells) were activated using anti-CD3/anti-CD28 Dynabeads (Invitrogen) (1:1 ratio) and IL-2 (2,000 U/ml) for three days in the presence or absence of human bone marrow-derived mesenchymal stem cells (hMSCs) at the ratio of 5:1 T cells:MSC. Expression of activation markers (CD25, CD69) was evaluated by flow cytometry and the mean fluorescence intensity (MFI) was shown for each marker. The values are representatives of three separate experiments.

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