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Figure 7 | Stem Cell Research & Therapy

Figure 7

From: Assessment of immunosuppressive activity of human mesenchymal stem cells using murine antigen specific CD4 and CD8 T cells in vitro

Figure 7

hMSCs inhibit secretion of Th1/Th2 cytokines by antigen stimulated murine T cells. Murine CD4+ (panel A) or CD8+ (panel B) T cells were cultured in the same conditions as described earlier (2 × 106 cells/well), together with their specific peptide and APCs. Human bone marrow-derived mesenchymal stem cells (hMCSs) were added to the T cells in the ratios 1:10 and 1:5 and supernatants were collected 72 hours later. Cytokine secretion was quantified and results are shown as pg/ml on the y axis. Shown are representative data out of two independent experiments with similar results. The samples were set up in triplicates in each experiment. Cytokine concentrations are represented in pg/ml for each sample, and results are expressed as the mean and SD of triplicates. *indicates P <0.05 for differences between conditions shown by the horizontal brackets.

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