Figure 8

Human fibrosarcoma cells do not affect mouse T cells proliferation and activation. Murine CD4⁺ T cells were labeled with CFSE and incubated with their cognate peptide and APCs for three days with or without human fibrosarcoma cells HT-1080. A total of 72 hours later, the cells were analyzed for the CFSE profile (A) and for the expression of two activation markers: CD25 (B) and CD69 (C). The unstimulated CD4⁺ T cells are represented by a red histogram, the stimulated T cells by a blue histogram and the cells that were co-cultured with human epithelial cells are represented as green (ratio 10:1) and orange (ratio 5:1) histograms. The results are representative of three independent experiments with similar results.