Figure 9
Effects of hMSCs on murine T cells are cell-contact dependent. A total of 2 × 106 CD4+ (panel A) or CD8+ (panel B) T cells were cultured on the bottom well of a transwell system together with the specific peptide and APCs. The human bone marrow-derived mesenchymal stem cells (hMSCs) were cultured in the upper well of the transwell system and the medium was shared between the two compartments. A total of 72 hours later the T cells were stained with antibodies against CD25, CD69 and CD62L and analyzed using a FACS Canto flow cytometer. Unstimulated T cells are represented by a red histogram, stimulated T cells by a blue histogram and T cells that were co-cultured with hMSCs are represented as green (ratio 10:1) and orange (ratio 5:1) histograms. The results are representative of three independent experiments, all with similar results.