Measures of brain area and evidence of integrity of the metabolic tissue in the striata of mice receiving umbilical cord mesenchymal stem cell transplantations. Gross morphology of the brain near the area of transplantation can be visualized with cytochrome oxidase labeling (A). Untreated R6/2 mice and mice that received transplantation of low-passaged UCMSCs had a significant decrease in total brain area when compared to WT mice (B). Optical densitometric measures of cytochrome oxidase in the striata (outlined in dashed line) revealed significantly less metabolic activity of striatal tissue in untreated R6/2 mice, when compared to WT mice at the time of necropsy (C). R6/2 mice that received transplantation of low- or high-passaged UC MSCs had significantly higher levels of metabolic activity in striatal tissue than did untreated R6/2 mice. (Note: *significant from WT; #significant from R6/2). Line graph represents mean value; error bars represent SEM. SEM, standard error of the mean; UCMSCs, umbilical cord mesenchymal stem cells; WT, wild type.