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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Aldehyde dehydrogenase and ATP binding cassette transporter G2 (ABCG2) functional assays isolate different populations of prostate stem cells where ABCG2 function selects for cells with increased stem cell activity

Figure 1

Isolation of ALDHHiand ALDHLowcells from prostate cells by ALDEFLUORâ„¢ assay. RWPE-1, RWPE-2, CWR-R1 and DU-145 prostate cells were stained with ALDEFLUORâ„¢ reagent and analyzed using BD FACS Aria II sorter. ALDHHi cells were detected in the higher fluorescent region (A, C, E and G). ALDHHi population was inhibited with DEAB resulting in no ALDHHi cell detection (B, D, F and H). The ALDHLow population is gated with a clear separation from the ALDHHi population.

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