Figure 5From: Aldehyde dehydrogenase and ATP binding cassette transporter G2 (ABCG2) functional assays isolate different populations of prostate stem cells where ABCG2 function selects for cells with increased stem cell activitySphere formation assay. RWPE-2, CWR-R1, and DU-145 prostate cells were treated in the presence of DEAB at 5, 10 and 20 μM or Ko143 at 1 and 5 μM concentration or vehicle control. Spheres were counted in each well after 10 to 14 days of growth. Sphere forming ability of RWPE-2 cells did not decrease upon treatment with DEAB (A). Sphere formation was decreased in RWPE-2 cells upon treatment with Ko143 at 5 μM concentration (B). Sphere formation was decreased in CWR-R1 cells upon treatment with DEAB (C) or Ko143 (D). Sphere forming ability of DU-145 cells did not decrease upon treatment with DEAB (E) or Ko143 (F).Back to article page