Figure 4

Effect of vWF on adhesion of hMSCs to collagen I coated or tissue culture treated plastic plates. Adhesion of hMSCs to collagen I coated and tissue culture plates was measured before and after immobilization of vWF. Before the adhesion assay vWF was removed and plates were washed with HBSS. Asterisks mark statistically significant differences compared to collagen I coated plate (t-test, P-value <0.05). Data are shown as mean ± SD of eight independent measurements. The insert shows vWF immobilization on collagen I coated (black circle) and tissue culture (white circle) plates exposed to 0 to 8 μg/ml vWF in HBSS for four hours. Immobilization of vWF was measured by ELISA and is shown in relative units (RU) as mean ± SD of eight independent measurements.