Figure 1

Schematic illustration of the heating method used. Heated medium was added to the wells in which human mesenchymal stem cells were cultured. The cells were kept on a hotplate at 37°C to replicate core body temperature. A hot probe was dipped into the medium to maintain the desired temperature. The medium was removed immediately once the desired time period had elapsed. DMEM+, Dulbecco’s modified Eagle’s medium supplemented with 20% fetal calf serum, penicillin and streptomycin.