Comparison of hMSC viability over 7 days. Trypan Blue exclusion dye was used to calculate the percentage of viable cells in the 48°C and 58°C groups. The detrimental effect on cell survival after exposure to 58°C is shown. However, cell number seems to recover between 3 and 7 days after heat treatment at 48°C/80 s and 48°C/150 s. Error bars cannot be seen here for those tests in which no dead cells were seen in any population i.e. the standard deviation = 0. ☆P≤0.05, heated groups vs. control kept at 37°C/5% CO2.