Figure 4
From: Evaluation of islets derived from human fetal pancreatic progenitor cells in diabetes treatment
Evaluation of insulin secretory function of differentiated cells in vitro . (A) Detection of insulin secretory granules. Insulin granules in the differentiated cells were detected by transmission electron microscopy (middle panel), with adult islet beta cells as positive control (left panel); the insulin distribution in differentiated cells was granular by immunofluorescence staining (right panel). (B) Insulin release in differentiated cells and islet-like structures upon glucose stimulation. (C) The time-effect relationship of insulin release in the response of differentiated cells to glucose. All the experiments were repeated three times, and the results are presented as means ± SD.