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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Efficient large-scale generation of functional hepatocytes from mouse embryonic stem cells grown in a rotating bioreactor with exogenous growth factors and hormones

Figure 1

Morphologic and histologic characteristics of EBs and EB-derived cells in the rotating bioreactor. (A) A rotating bioreactor was used to create a 3D RCCS for mES cell culture. (B) A large number of homogeneous EBs was formed at day 7 in a rotating bioreactor with exogenous growth factors and hormones. (C) Image of EBs by stereomicroscopy at day 14. (D-F) Images of EBs with H&E staining at day 7 (D), 14 (E), 21 (F). EB-derived cells from day 14 and day 21, but not day 7, exhibited polyhedral contours, large nuclei, and a cordlike arrangement. (G-I) Images of EB-derived cells. When the day 21 EB-derived cells were seeded in six-well plates (G), most of the cells had a uniform pyramidal shape after 3 days (H) and 5 days (I) of culture. Scale bar, 100 μm.

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Stem Cell Research & Therapy

ISSN: 1757-6512