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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Efficient large-scale generation of functional hepatocytes from mouse embryonic stem cells grown in a rotating bioreactor with exogenous growth factors and hormones

Figure 5

Cytochrome P450 activity in EBs and EB-derived cells. (A-C) PROD activity (red) was detected in day-21 EBs (A), day-21 EB-derived cells grown in monolayer culture (B), and mES cells differentiated in 2D culture (C) by CLSM. Scale bar, 100 μm. (D-F) Flow cytometric analysis showed that the number of PROD-positive day-21 EB-derived cells (D) was higher than the number of differentiated mES cells from the 2D culture (E). A significant difference in the number of PROD-positive cells was noted between the differentiated cell samples from the 3D and 2D cultures (F). *P < 0.05.

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Stem Cell Research & Therapy

ISSN: 1757-6512