Skip to main content
Figure 6 | Stem Cell Research & Therapy

Figure 6

From: Efficient large-scale generation of functional hepatocytes from mouse embryonic stem cells grown in a rotating bioreactor with exogenous growth factors and hormones

Figure 6

LDL-uptake analysis of EBs and EB-derived cells. (A-C) CLSM images indicated that the day-21 EBs (A) could incorporate DiI-Ac-LDL (red). Additionally, more day 21 EB-derived cells grown in monolayer culture incorporated DiI-Ac-LDL (B) than the mES cells differentiated in 2D culture (C). Scale bar, 100 μm. (D-F) Flow-cytometric analysis showed that the number of LDL uptake-positive cells derived from day-21 EBs (D) was higher than the number of differentiated mES cells from 2D culture exhibiting LDL uptake (E). A significant difference in the LDL uptake between the differentiated cell samples from the 3D and 2D cultures (F). *P < 0.05.

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512