Stemness features of ScI- and BFP-pASCs. Cell proliferation expressed as doubling time (DT, hours) of ASCs from II to IV passage (A). Viability assessed by MTT assay at days 1, 3, and 7 (B). Morphology of ScI-pASCs and BFP-pASCs (optical microscopy, 200× magnification; scale bar, 100 μm) (C). Clonogenicity from passage I to IV expressed as colony-forming units (CFU-F) percentage (ratio of number of colonies/number of plated cells × 100) (D, upper panel). Data are expressed as mean ± SEM (n = 6). Representative ScI-pASCs and BFP-pASCs plates stained with crystal violet (D, lower panel).