Influence of biomaterials on pASC osteogenic differentiation. Calcified ECM deposition in undifferentiated (CTRL, white bars) and osteogenic-differentiated (OSTEO, dark bars) ScI- and BFP-pASCs, cultured for 21 days on monolayer (plastic adherent, PA), or seeded on titanium disks (TIT) or on silicon carbide–plasma-enhanced chemical vapor deposition (SIC) fragments (A). Protein concentration of pASCs cultured either in monolayer or adhering to biomaterial both in CTRL (white row) and OSTEO (dark row) is reported in panel (B). Data are expressed as mean ± SEM (n = 3). OSTEO versus CTRL: **P < 0.01; TIT versus PA; §P < 0.05; §§P < 0.01; §§§P < 0.001.