Human jejunal enteroids support rotavirus growth. (a) Nonstructural viral proteins, NSP4 (green) and NSP5 (red), were detected in enteroids by confocal microscopy. Basolateral membrane marked by E-cadherin (purple) and nuclei (blue) by 4',6-diamidino-2-phenylindole (DAPI) staining. (b) GAPDH-normalized rotavirus VP7 RNA levels are shown as fold increases relative to 0 hours post infection. Values are means of triplicates and standard deviation for three amplifications per sample.