Figure 3
From: Toward a 3D model of human brain development for studying gene/environment interactions
Three-dimensional aggregates formed from cultures pre-differentiated in two dimensions for 4 weeks. Cells were differentiated in adherent culture for 28 days in differentiation medium. After that cells were re-suspended and differentiated in suspension in a constant movement until 56 days. Phase contrast images show (a) human neural progenitor cell (hNPC) adherent cultures, (b) cultures after 4 weeks of pre-differentiation in two dimensions, (c) aggregates obtained from cultures pre-differentiated in two dimensions for 4 weeks. The lower panel shows the relative expression of different neuronal markers (β-tubulin III (Tubb3), neurofilament heavy chain (Nefh), tyrosine hydroxylase (Th), synapsin 1 (Syn1)) during differentiation after 0, 35 and 56 days. *Statistically different from DIV 0, P <0.001 Student's t test.