Figure 2
From: Three-dimensional osteochondral microtissue to model pathogenesis of osteoarthritis
Schematic of workflow and milestones in developing a functional endochondral microtissue. (A) to (F) Specific methodologies required are described. See text for details. cc, chondrocyte construct; ec, endothelial construct; hf, human fibroblasts; lc, lower medium conduit; mc, mesenchymal construct; oc, osteoblast construct; p, platform; sf, synovial fluid; uc, upper medium conduit; a1, lower adapter; a2, upper adapter. IHC, immunohistochemistry; mCT, microcomputed tomography; MMP, matrix metalloproteinases; MRI, magnetic resonance imaging; MSC, mesenchymal stem cell; MTS, 3 (4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium.