Production of respiratory tissue from murine embryonic stem cells on whole acellular trachea-lung scaffold. (A) Staining control, 4',6-diamidino-2-phenylindole (DAPI)-blue. (B), (C) Production of ciliated epithelial cells in the lower trachea. (D) Staining control for (E). (E) Production of Clara cells in upper bronchi of whole recellularized trachea-lung. CC10, Clara cell protein 10. (F) Staining control for (G). (G) Production of cells expressing surfactant protein A (SPA) indicating the presence of type II alveolar epithelial cells. Modified version of a figure adapted from Cortiella and colleagues .