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Table 2 Comparison of biological parameters, according to smoking status

From: Difference in mobilization of progenitor cells after myocardial infarction in smoking versus non-smoking patients: insights from the BONAMI trial

Cell type

Bone marrow

Peripheral blood

Mean ± SD (number)

P-valuec

Mean ± SD (number)

P-valuec

Non smokers and former smokers

Active smokers at admission

Non smokers and former smokers

Active smokers at admission

White cells (WC)

      

 Leukocytes/μL

10,685 ± 4,818 (20)

20,029 ± 9,943 (27)

0.001

7,904 ± 2,249 (41)

9,171 ± 2,426 (52)

0.016

Haematopoietic progenitor cells

      

 CD45dimCD34+/μL

49.0 ± 29.8 (20)

83.1 ± 52.2 (28)

0.02

2.7 ± 2.6 (40)

2.8 ± 2.0 (50)

NS (0.9)

 CD45dimCD34+/105 WC

399.3 ± 145.4 (20)

419.0 ± 158.9 (27)

NS (0.9)

33.7 ± 26.1 (40)

30.8 ± 20.0 (50)

NS (0.7)

 CD45dimCD34 + CD133+/μL

41.8 ± 24.4 (19)

79.6 ± 52.0 (28)

0.005

2.6 ± 2.6 (39)

2.7 ± 1.9 (50)

NS (0.3)

 CD45dimCD34 + CD133+/105 WC

394.7 ± 148.8 (19)

402.0 ± 148.5 (27)

NS (0.4)

32.9 ± 26.0 (39)

29.4 ± 19.3 (50)

NS (0.7)

 CD45dimCD34 + CXCR4+/μL

43.6 ± 25.7 (19)

82.6 ± 52.9 (28)

0.01

2.6 ± 2.6 (39)

2.7 ± 2.0 (49)

NS (0.5)

 CD45dimCD34 + CXCR4+/105 WC

392.1 ± 144.1 (19)

405.5 ± 165.5 (27)

NS (0.9)

32.7 ± 25.6 (39)

29.5 ± 19.7 (49)

NS (0.7)

Endothelial progenitor cells

      

 CD45dimCD34 + KDR+/μL

1.5 ± 0.7 (16)

1.8 ± 1.6 (22)

NS (0.9)

0.2 ± 0.1 (31)

0.1 ± 0.2 (40)

0.02

 CD45dimCD34 + KDR+/105 WC

13.5 ± 7.1 (16)

9.1 ± 8.2 (21)

0.04

2.2 ± 1.9 (31)

1.2 ± 1.8 (40)

0.005

Colony-forming cell assays

      

 CFU-GM/1.105 BMMC or /2.105 PBMC

68.5 ± 54.5 (16)

95.7 ± 68.7 (22)

NS (0.2)

13.6 ± 16.6 (39)

18.6 ± 27.8 (53)

NS (0.4)

 CFU-EC/1.106 BMMC or /2.106 PBMC

3.9 ± 5.3 (15)

11.6 ± 15.0 (22)

NS (0.1)

3.6 ± 5.7 (34)

2.6 ± 4.4 (50)

NS (0.6)

Migration assay

      

 Spontaneous migrationa

31,374 ± 8,731 (16)

30,877 ± 8,600 (24)

NS (0.8)

38,613 ± 15,212 (38)

35,383 ± 10,607 (48)

NS (0.4)

 G-CSF 100 ng/mL

1.5 ± 0.4 (16)

1.3 ± 0.4 (24)

0.03

1.3 ± 0.4 (38)

1.4 ± 0.3 (48)

NS (0.3)

 SDF-1α 100 ng/mLb

1.3 ± 0.3 (16)

1.36 ± 0.4 (24)

NS (0.9)

1.28 ± 0.3 (38)

1.31 ± 0.3 (48)

NS (0.6)

 VEGF 100 ng/mLb

1.32 ± 0.3 (16)

1.49 ± 0.5 (24)

NS (0.3)

1.3 ± 0.3 (38)

1.36 ± 0.4 (48)

NS (0.3)

VEGF

      

 Plasmatic VEGF (pg/mL)

-

-

-

239.5 ± 359.3 (38)

204.7 ± 341.3 (45)

NS (0.6)

CRP

-

-

-

23.7 ± 27.5 (26)

28.0 ± 35.7 (39)

NS (0.07)

  1. anumber of cells that migrated without cytokines; bratio between the number of cells that migrated in cytokine presence and the spontaneous migration; cMann–Whitney test. BMMC, bone marrow mononuclear cell; CFU-GM, granulocyte macrophage colony forming units; CRP, C-reactive protein; G-CSF, granulocyte colony stimulating factor; NS, not significant; SD, standard deviation; SDF-1α, stromal cell-derived factor-1α; VEGF, vascular endothelial growth factor.
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Stem Cell Research & Therapy

ISSN: 1757-6512