Figure 1

Flow cytometric results of cellular apoptosis and Western blot results of oxidative stress. A) Compares the incidence of early (annexin V+/PI-) and late (annexin V+/PI+) phases of apoptosis between healthy adipose-derived mesenchymal stem cells (H-ADMSC) and apoptotic (A) (that is, 96 hours of serum-free cell culture) ADMSC (A-ADMSC). B) For A-ADMSCs, early apoptosis and late apoptosis increased at 24 hours and further increased by 48 hours after re-culture (after 96 hours of serum-free cell culture) in normal culture medium with adequate nutrition. * versus H-ADMSC in early or late phase of cellular apoptosis, P <0.0001. C) DAPI stain (nuclei were stained in blue color) indicated the cell apoptosis (white arrows). Apoptosis (white arrows) in A-ADMSC was markedly increased at 48 hours after being re-cultured in normal culture medium with adequate nutrition. D and E) Oxidized protein in lung (D) and kidney (E) at 72 hours after cecal ligation and puncture (CLP) procedure. DNP, 1–3 dinitrophenylhydrazone; M.W., molecular weight (Note: The right lane and left lane shown on the upper panel represent control oxidized molecular protein standard and protein molecular weight marker, respectively). 1, sham control (SC); 2, cecal ligation and puncture (CLP); 3, CLP + H-ADMSC; 4, CLP + A-DMSC. * versus other groups with different symbols, P <0.001. Statistical analysis using one-way ANOVA, followed by the Bonferroni multiple comparison post hoc test (n = 8). Symbols (*, †, ‡) indicate significant difference (<0.05). ANOVA, analysis of variance; DAPI, 4',6-diamidino-2-phenylindole.