Figure 11

Histopathological changes and inflammatory cell infiltration in lung parenchyma at 72 hours after the CLP procedure. A to D) Microscopic findings of H & E stain (100x) of lung parencyma at 72 hours after the procedure. Scale bars in the right lower corners represent 100 μm. E) Quantitative analysis of the number of alveolar sacs. * versus other groups with different symbols (*, †, ‡, §), P <0.0001. F) Quantitative analysis of crowded score. * versus other groups with different symbols (*, †, ‡, §), P <0.001. G to J) Immunohistochemical stain (200x) for CD3+ cells in lung parencyma at 72 hours after the procedure. Scale bars in right lower corners represent 50 μm. K) Quantitative analysis of CD3+ cells. * versus other groups with different symbols (*, †, ‡, §), P <0.0001. All statistical analyses using one-way ANOVA, followed by the Bonferroni multiple comparison post hoc test (n = 8). Symbols (*, †, ‡, §) indicate significance (at 0.05 level). SC, sham control; CLP, cecal ligation and puncture; H-ADMSC, healthy adipose-derived mesenchymal stem cell; A-ADMSC, apoptotic adipose-derived mesenchymal stem cell. ANOVA, analysis of variance.