Figure 13

Histopathological changes in lung and kidney at 72 hours after the CLP procedure with one dose of ADMSC treatment. A to D) Microscopic findings of H & E stain (100x) of lung parenchyma at 72 hours after the procedure. Scale bars in the right lower corners represent 100 μm. E) Quantitative analysis of the number of alveolar sacs. * versus other groups with different symbols (*, †, ‡, §), P <0.001. F) Quantitative analysis of crowded score. * versus other groups with different symbols (*, †, ‡, §), P <0.001. G to J) H & E stain (200x) showing the pathological findings in kidney at 72 hours after the CLP procedure. Scale bars in the right lower corners represent 50 μm. yellow arrows = loss of brush border in renal tubules; blue arrows = dilatation of Bowman’s capsule. K) * versus other groups with different symbols (*, †, ‡, §), P <0.001. All statistical analyses using one-way ANOVA, followed by the Bonferroni multiple comparison post hoc test (n = 6). Symbols (*, †, ‡, §) indicate significance (at 0.05 level). SC, sham control; CLP, cecal ligation and puncture; H-ADMSC, healthy adipose-derived mesenchymal stem cell; A-ADMSC ,apoptotic adipose-derived mesenchymal stem cell. ANOVA, analysis of variance.