Figure 14

Immunofluorescent staining for identifying infiltration of CD68+ cells in lung and kidney at 72 hours after the CLP procedure with one dose of ADMSC treatment. A to D) and F to I) IF microscopic findings (200x) of the number of CD68+ cells (white arrows indicate CD68+ cells) in lung and kidney parenchyma, respectively. Scale bars in the right lower corners represent 50 μm. E) and J) Quantitative analysis of the number of CD68+ cells in lung and kidney, respectively. Yellow arrows indicated the ADMSCs. * versus other groups with different symbols (*, †, ‡, §), P <0.0001. All statistical analyses using one-way ANOVA, followed by the Bonferroni multiple comparison post hoc test (n = 6). Symbols (*, †, ‡, §) indicate significance (at 0.05 level). SC, sham control; CLP, cecal ligation and puncture; H-ADMSC, healthy adipose-derived mesenchymal stem cell; A-ADMSC, apoptotic adipose-derived mesenchymal stem cell. ANOVA, analysis of variance.