Figure 8
The protein expression of mitochondrial integrity in both lung and kidney and DNA damage marker in kidney at 72 hours after the CLP procedure. A and B) The protein expression of mitochondrial cytochrome C (Mito-Cyt C) in lung (A) and kidney (B). For lung: * versus other groups with different symbols, P <0.01. For kidney: * versus other groups with different symbols, P <0.001. C and D) The protein expression of mitochondrial cytochrome C (Cyto-Cyt C) in lung (C) and kidney (D). For lung: * versus other groups with different symbols, P <0.001. For kidney: * versus other groups with different symbols, P < 0.01. E to H) Immunofluorescent stain (400x) for γ-H2AX + cells (white arrows) in kidney. I) Quantitative analysis of γ-H2AX + cells (%), P <0.0001). DAPI stain for identification of nuclei (blue color). Scale bars in the right lower corner represent 20 μm. Statistical analysis using one-way ANOVA, followed by the Bonferroni multiple comparison post hoc test (n = 8). Symbols (*, †, ‡, §) indicate significant difference (<0.05). SC, sham control; CLP, cecal ligation and puncture; H-ADMSC, healthy adipose-derived mesenchymal stem cell; A-ADMSC, apoptotic adipose-derived mesenchymal stem cell. ANOVA, analysis of variance; DAPI, 4',6-diamidino-2-phenylindole.