Figure 1
From: Inhibition of mesenchymal stromal cells by pre-activated lymphocytes and their culture media
Impedance profiles and mean CI values of MSCs in co-culture with PBMCs. Unstimulated (NS) PBMCs (A) and PHA-stimulated PBMCs (B) were added to MSCs (400/well) after about 72 hours of culture at four different ratios. The experiment was conducted in triplicate. (C) The mean CI values, obtained in the four independent experiments, treating MSCs with resting or pre-activated PBMCs. Results were analyzed and expressed as percentage of the untreated MSCs and presented as mean values ± standard deviations. Statistical analysis was performed using one-way ANOVA (*P <0.05; **P <0.01; ***P <0.001). ANOVA, analysis of variance; CI, cell index; MSCs, mesenchymal stem cells; PBMCs, peripheral blood mononuclear cells.