Figure 4
From: Inhibition of mesenchymal stromal cells by pre-activated lymphocytes and their culture media
Impedance profiles and mean CI values of MSCs treated with lymphocyte conditioned medium (CM). MSCs were stimulated with CM from unstimulated lymphocytes (A) and PHA-stimulated lymphocytes (B). The CM was added to MSCs (400/well) at four different dilutions after about 72 hours of culture. The experiment was conducted in triplicate. (C) The mean CI values, obtained in the four independent experiments, treating MSCs with CM from resting or pre-activated PBMCs. Results were analyzed and expressed as percentage of the untreated MSCs and presented as mean values ± standard deviations. Statistical analysis was performed using one-way ANOVA (*P <0.05; **P <0.01). ANOVA, analysis of variance; CI, cell index; MSCs, mesenchymal stem cells; PBMCs, peripheral blood mononuclear cells; PHA, phytohemagglutinin.