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Figure 6 | Stem Cell Research & Therapy

Figure 6

From: Inhibition of mesenchymal stromal cells by pre-activated lymphocytes and their culture media

Figure 6

MTT cell viability assay. Cell viability was assessed after MSC treatment with the indicated dose of lymphocyte CM. (A) CM from unstimulated PBMCs; (B) CM from PHA-stimulated PBMCs. Results were analyzed and expressed as percentage of the control (untreated MSCs) and presented as mean values ± standard deviations of the three independent experiments performed in duplicate. Statistical analysis was performed using Student’s t test (*P <0.05; **P <0.01). CM, conditioned medium; MSCs, mesenchymal stem cells; MTT, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; PBMCs, peripheral blood mononuclear cells; PHA, phytohemagglutinin.

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