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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Sera of overweight people promote in vitro adipocyte differentiation of bone marrow stromal cells

Figure 1

Experimental plan. Bone marrow was collected from healthy patients and mononuclear cell fractions were used to provide bone marrow stromal cultures containing MSCs. Cultures were propagated for seven to ten days. Then cultures were treated with OS and HS for three days (priming). At the end of priming, apopotosis and senescence were evaluated. Cultures were then incubated in adipogenic or osteogenic differentiation media for 15 days and the differentiation processes were evaluated. HS, healthy weight sera; MSCs, mesenchymal stem cells; OS, overweight sera.

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