Cytokine and reactive oxygen species (ROS) detection in sera. A) Arrays incubated with HS and OS samples. The table below the arrays shows the name and the relative position on the Panomics TranSignal Human Cytokine Antibody Array of the cytokines that were detected in OS and HS sera. On the table ‘Positive’ and ‘Negative’ are the array internal controls. Array signals were acquired using the Chemidoc system (Bio-Rad) and the associated software QuantityOne. The graph shows the cytokine expression levels in the OS and HS sera. Data are expressed as arbitrary units (*P < 0.05). B) The table shows the expression of ROS in HS and OS samples. Data are expressed in arbitrary units (± SD, number of experiment replicates: three). HS, healthy weight sera; OS, overweight sera.