| Subset | Marker | Company | Clone | Sec Ab | Dilution | Positive control |
---|---|---|---|---|---|---|---|
Multicolor FCM | 1 | CD29-Alexa488+ | Biolegend | TS2/16 | Â | 1:20 | MNCs |
MHC-II− | Serotec | CVS20 | RPE | 1:50 | MNCs | ||
7-AAD− | Calbiochem |  |  |  |  | ||
CD44-APC+ | BD | IM7 | Â | 1:20 | MNCs | ||
2 | CD105-RPE+ | Serotec | SN6 | Â | 1:10 | Endothelial cells | |
7-AAD− | Calbiochem |  |  |  |  | ||
CD90+ | VMRD | DH24A | Alexa647 | 1:100 | MNCs | ||
3 | CD45-Alexa488− | Serotec | F10-89-4 |  | 1:5 | MNCs | |
CD73+ | Abcam | 10f1 | RPE | 1:5 | Lymphocytes | ||
7-AAD− | Calbiochem |  |  |  |  | ||
4 | Monocyte-Alexa488− | Serotec | MAC387 |  | 1:2.5 | MNCs | |
CD79α-Alexa647− | Serotec | HM57 |  | 1:2.5 | MNCs | ||
Single-color FCM | Â | MHC-I+ | VMRD | PT85A | RPE | 1:66 | MNCs |
Secondary Ab | 1 and 3 | Sheep anti-mouse IgG-RPE | Sigma-Aldrich | Â | Â | 1:20 | Â |
 | 2 | Goat anti-mouse IgG-Alexa647 | Invitrogen Corporation |  |  | 1:200 |  |
Isotype controls | 1 and 4 | Mouse IgG1-Alexa488 | Biolegend | Â | Â | 1:20 | Â |
 | 1-3 | Mouse IgG1-RPE | Biolegend |  |  | 1:10 |  |
 | 1 | Rat IgG2b-APC | Biolegend |  |  | 1:20 |  |
 | 2 | Mouse IgM | BD |  | Alexa647 | 1:50 |  |
 | 3 | Mouse IgG2-Alexa488 | Biolegend |  |  | 1:20 |  |
 | 4 | Mouse IgG1-Alexa647 | Biolegend |  |  | 1:100 |  |