Characterization and profiling of immunomodulatory genes of equine mesenchymal stromal cells from non-invasive sources

De Schauwer, Catharina; Goossens, Karen; Piepers, Sofie; Hoogewijs, Maarten K; Govaere, Jan LJ; Smits, Katrien; Meyer, Evelyne; Van Soom, Ann; Van de Walle, Gerlinde R

doi:10.1186/scrt395

Stem Cell Research & Therapy

Table 2 Primer sequences used for the real-time polymerase chain reaction studies

From: Characterization and profiling of immunomodulatory genes of equine mesenchymal stromal cells from non-invasive sources

	Gene	Name	Primer sequence 5′ → 3′	Amplicon size, bp	Ta, °C	Efficiency, %	Correlation
Reference genes	ACTB	Beta actin	CCAGCACGATGAAGATCAAG	88	60	98	0.998
	ACTB	Beta actin	GTGGACAATGAGGCCAGAAT	88	60	98	0.998
	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	CAGAACATCATCCCTGCTTC	187	59	100	0.998
	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	ATGCCTGCTTCACCACCTTC	187	59	100	0.998
	H2A	Histone H2A type 1-C	ATATTCAGGCCGTGCTGCT	105	60	100	0.999
	H2A	Histone H2A type 1-C	TTTGGGTTTCAAAGCGTTTC	105	60	100	0.999
	HPRT1	Hypoxanthine phosphoribosyl-transferase 1	GGCAAAACAATGCAAACCTT	163	57	100	0.994
	HPRT1	Hypoxanthine phosphoribosyl-transferase 1	CAAGGGCATATCCTACGACAA	163	57	100	0.994
	SDHA	Succinate dehydrogenase complex, subunit A	TCCATCGCATAAGAGCAAAG	159	59	99	0.997
	SDHA	Succinate dehydrogenase complex, subunit A	GGTGGAACTGAACGAACTCC	159	59	99	0.997
	RPL32	Ribosomal protein L32	AGCCATCTACTCGGCGTCA	149	60	100	0.995
	RPL32	Ribosomal protein L32	TCCAATGCCTCTGGGTTTC	149	60	100	0.995
	UBC	Ubiquitin C	GCAAGACCATCACCCTGGA	206	60	97	0.996
	UBC	Ubiquitin C	CTAACAGCCACCCCTGAGAC	206	60	97	0.996
	TUBA4A	Tubulin, alpha 4a	GCCCTACAACTCCATCCTGA	78	60	100	0.999
	TUBA4A	Tubulin, alpha 4a	ATGGCTTCATTGTCCACCA	78	60	100	0.999
Test genes	CD40	Cluster of differentiation 40	CAGGAAAGAAACTGGTGAATG	180	62	106	0.993
	CD40	Cluster of differentiation 40	AAGTGGCGTCTGTTGTTGAG	180	62	106	0.993
	CD80	Cluster of differentiation 80	CACCTTCACCGACATCACC	106	62-64	102	0.995
	CD80	Cluster of differentiation 80	AGACCCCCTTTCGCTCTTC	106	62-64	102	0.995
	CD86	Cluster of differentiation 86	AGTATAAAGGCCGCACAAGC CCTTGGGTAGATGAGCAGGT	247	63	95	0.990
	HGF	Hepatocyte growth factor	TGCATTCAAGGTCAAGGAGA TTTTGGAATTTGGGAGCAGT	234	63	84	0.983
	IDO	Indoleamine 2,3-dioxygenase	ACAACATCAGGACCAGGACAC TCCAGACGCCTTCATAGAG	198	61-64	72	0.957
	TGFβ	Transforming growth factor-β	GGAATGGCTGTCCTTTGATG CGGAGTGTGTTATCTTTGCTGTC	120	61-64	92	0.997
	TNFα	Tumor necrosis factor-α	GCCTCAGCCTCTTCTCCTTC GGCTTGTCACTTGGGGTTC	172	62-64	113	0.999

Ta, annealing temperature.

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ISSN: 1757-6512

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