Figure 5

Ad-MSCs-SF and D-Ad-MSCs-SF release angiogenic factors that stimulate migration of HUVECs. At the end of the HUVECs scratch test, aliquots of CM were collected. The presence of human VEGF (A), FGF2 (B), TGFβ (C) and EGF (D) were quantified (pg/mL) by ELISA in accordance with the standard guideline protocol supplied with the kits. The background value (<10%) of each growth factor analyzed and contained in SCM not cultured with cells was subtracted. The results were normalized for the growth factor release by HUVEC co-cultured with untreated SF patches. Data are expressed as mean ± SD of the secreted factor. The assay was repeated twice and each sample was run in triplicate. *P <0.05; **P <0.01 versus HUVECs cultured in the presence of untreated SF patches. Ad-MSCs-SF, silk fibroin patch cellularized with human adipose-derived mesenchymal stromal cells; CM, conditioned medium; D-Ad-MSCs-SF, silk fibroin patch after human adipose-derived mesenchymal stromal cells removal (decellularization); EGF, epidermal growth factor; FGF2, fibroblast growth factor 2; HUVECs, human umbilical vein endothelial cells; SCM, stem cell medium; SF, silk fibroin; TGFβ, transforming growth factor β: VEGF, vascular endothelial growth factor.